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KMID : 0438219730100030747
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Korea University Medical Journal
1973 Volume.10 No. 3 p.747 ~ p.756
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The Effect of Temporary Renal Circulatory Stasis on Glucose Metabolism in Rabbit Kidney
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Abstract
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This study was designed to observe whether the metabolic capacity of renal tissue could be reduced by the effects of a period of induced ischemia. Metabolic studies were performed on slices of rabbit renal cortex, outer medulla, inner medulla and papillary tip which were incubated in Krebs-Ringer bicarbonate buffer solution.
Ischemia was induced by occluding the renal artery or renal vein of the left kidney for an hour, thereafter the occlusion was removed for 30 minutes. The right kidney used as a paired control. Analyses were performed on final media after removal of slices incubation. Glucose was measured by the method of Nelson after deproteinization according to Somogyi, and medium lactate was determined according to the method of Barker and Summerson. The rates of oxygen consumption (QO₂) were measured manometrically in a Warburg apparatus using standard manometric techniques as described by Umbreit et al.
The results are summarized as follows:
1. Renal venous occlusion has a more pronounced effect on kidney weight increment than does renal arterial occlusion.
2. Arterial occlusion increases the rate of lactate production significantly in all kidney regions than venous occlusion.
3. Occlusion of either the renal vein or artery reducest he rate of oxygen consumption of all regions in glucose free medium.
4. The reduced rate of oxygen consumption following renal arterial occlusion recovered completely in glucose added medium.
5. Both cortex and medulla appear to be more sensitive to ischemia induced by renal venous occlusion than by renal arterial occlusion.
It is inferred from the above results that, at medium oxygen level approximating that believed to exist in vivo, glycolysis in papillary tip, mixed glycolysis and oxidation in inner medulla and outer medulla, and oxidative metabolism in cortex are the major energy-yielding pathways in rabbit kidney.
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